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KMID : 1234920140140020047
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Journal of Korean Oriental Association for Study of Obesity
2014 Volume.14 No. 2 p.47 ~ p.54
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Anti-Oxidative and Anti-Obesity Effect of Combined Extract and Individual Extract of Samjunghwan
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Han Kyung-Sun
Wang Jing-Hua
Lim Dong-Woo
Chin Young-Won
Choi Young-Hee
Choi Han-Seok
Lee Myeong-Jong
Kim Ho-Jun
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Abstract
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Objectives: This study is to confirm the effect of combined extract and individual extract of Samjunghwan (SJH) in anti-oxidative and anti-obesity effect.
Methods: Combined ethanol extract of readily made SJH and individual ethanol extract of Atractylodes japonica, Cortex lycii radicis, and Morus alba Linne was combined after the extraction. To evaluate the anti-oxidative effect of SJH, total phenol compound and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability were conducted. Real-time quantitative-polymerase chain reaction analysis of transcription factor peroxisome proliferator-activated receptror γ (PPARγ), adenosine monophosphate-activated protein kinase (AMPK)-α1, tumor necrosis factor-α (TNFα) and 3-hydroxy-3-methylglutaryl CoA reducatase (HMG-CoA reductase) were done with 3T3-L1 cells to investigate the ant-obesity effect. Also, cell viability analysis were done to see to toxicity of SJH.
Results: Individual extract of SJH showed significant decrease in TNFα and AMPK transcription while PPARγ showed significant increase. Combined extract and individual extract of SJH both showed decrease in HMG-CoA reductase. DPPH free radical scavenging ability and total phenol compound was analogous between two groups.
Conclusions: Individual extract of SJH appears to be more effective in anti-oxidation and anti-obesity effect compared to combined extract of SJH.
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KEYWORD
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Samjunghwan, Obesity, Antioxidants
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